JAK3 (781-end), active, GST tagged human
SIGMA/SRP5041 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
Product Type: Chemical
SDS-PAGE JAK3 (781-end), active, GST tagged, human: Cat. No. SRP5041: Purity was determined by densitometry. Molecular weight ~64 kDa. See CofA for lot-specific data.
Specific Activity JAK3 (781-end), active, GST tagged, human: Cat. No. SRP5041: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~64 kDa |
| NCBI accession no. | NM_000215   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 77-104 nmol/min·mg |
| storage temp. | −70°C |
| General description: | JAK3 is a member of the JAK family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. Low levels of JAK3 expression is detected in immature hematopoietic cells, which dramatically increases during terminal differentiation of these cells suggesting a role of JAK3 in the differentiation of hematopoietic cells. Mutations in JAK3 are associated with autosomal SCID (severe combined immunodeficiency disease). Mice lacking JAK3 show a severe block in B-cell development at the pre-B stage in bone marrow suggesting that JAK3 is critical for the progression of B-cell development in the bone marrow. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 77-104 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |