MARK2, active, GST tagged human
SIGMA/SRP5045 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: EMK1; MGC99619; PAR-1
Product Type: Chemical
SDS-PAGE MARK2, active, GST tagged, human: Cat. No. SRP5045: Purity was determined by densitometry. Molecular weight ~114 kDa. See CofA for lot-specific data.
Specific Activity MARK2, active, GST tagged, human: Cat. No. SRP5045: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~114 kDa |
| NCBI accession no. | NM_001039469   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 676-914 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MARK2 or microtubule affinity-regulating kinase 2 is a member of the Par-1 family of serine/threonine protein kinases. MARK2 is an important regulator of cell polarity in epithelial and neuronal cells and controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. MARK2 is a key target of H. pylori CagA in the disorganization of gastric epithelial architecture underlying mucosal damage, inflammation, and carcinogenesis. MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. MARK2 function in the establishment of T cell polarity following engagement to an APC. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 676-914 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |