MELK (1-340), active, GST tagged human
SIGMA/SRP5048 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HPK38; KIAA0175
Product Type: Chemical
SDS-PAGE MELK (1-340), active, GST tagged, human: Cat. No. SRP5048: Purity was determined by densitometry. Molecular weight ~61 kDa. See CofA for lot-specific data.
Specific Activity MELK (1-340), active, GST tagged, human: Cat. No. SRP5048: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~61 kDa |
| NCBI accession no. | NM_014791   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 170-230 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MELK or maternal embryonic leucine zipper kinase is a member of the CAMKL kinase family. MELK is a key regulator of the proliferation of malignant brain tumors including their stem cells. MELK transcript abundance correlates with malignancy grade in human astrocytomas and represents a therapeutic target for the management of the most frequent brain tumors in adult and children. MELK also plays a role in mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL. Therefore, the kinase activity of MELK could be a promising molecular target for development of therapy for patients with breast cancers. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 170-230 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |