NDR, active, GST tagged human
SIGMA/SRP5050 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: NDR1; STK38
Product Type: Chemical
SDS-PAGE NDR, active, GST tagged, human: Cat. No. SRP5050: Purity was determined by densitometry. Molecular weight ~81 kDa. See CofA for lot-specific data.
Specific Activity NDR, active, GST tagged, human: Cat. No. SRP5050: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~81 kDa |
| NCBI accession no. | NM_007271   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 3.4-4.6 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NDR or Nuclear Dbf2-related protein is a serine/threonine kinase in the subgroup of the AGC kinase family that is regulated by Mob proteins. Fas and TNFα receptor stimulation activates human NDR by promoting phosphorylation at the hydrophobic motif. NDR acts as a novel pro-apoptotic kinase, and is a key member of the RASSF1A/MST1 signaling cascade. NDR is crucial for the fidelity of mitotic chromosome alignment in mammalian cells. NDR is incorporated into the HIV-1 particles and is cleaved by the HIV-1 protease. NDR-driven centrosome duplication requires Cdk2 activity, and Cdk2-induced centrosome amplification is affected upon reduction of NDR activity. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 3.4-4.6 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |