NEK11, active, GST tagged human
SIGMA/SRP5051 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: FLJ23495
Product Type: Chemical
SDS-PAGE NEK11, active, GST tagged, human: Cat. No. SRP5051: Purity was determined by densitometry. Molecular weight ~110 kDa. See CofA for lot-specific data.
Specific Activity NEK11, active, GST tagged, human: Cat. No. SRP5051: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~110 kDa |
| NCBI accession no. | NM_024800   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 21-29 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NEK11 is a member of the NEK family and the protein localizes to the nucleoli where it may function with NEK2A in the S-phase checkpoint. NEK2 phosphorylates NEK11 in its C-terminal autoinhibitory domain and elevates NEK11 kinase activity by dissociating the autoinhibitory domain from the N-terminal catalytic domain. NEK11 protein appears to play a role in DNA replication and response to genotoxic stress. NEK11 activity is activated in HeLa cells treated with various DNA-damaging agents and replication inhibitors. Activation of NEK11 by genotoxic agents is suppressed by inhibitor of ATR/ATM-mediated signalling indicating that NEK11 has a role in S-phase checkpoint downstream of ATR/ATM signalling. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 21-29 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |