NEK9 (1-979), (deleted fragment 346-731), active, GST tagged human
SIGMA/SRP5053 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: DKFZp434D0935; MGC138306; MGC16714; NEK8; NERCC; NERCC1
Product Type: Chemical
SDS-PAGE NEK9 (1-979), (346-731), active, GST tagged, human: Cat. No. SRP5053: Purity was determined by densitometry. Molecular weight ~115 kDa. See CofA for lot-specific data.
Specific Activity NEK9 (1-979), (346-731), active, GST tagged, human: Cat. No. SRP5053: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~115 kDa |
| NCBI accession no. | NM_033116   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 102-138 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. NEK9 can catalyze the phosphorylation of recombinant NEK6 and NEK7 in vitro leading to its activation. This suggests that NEK9 may be responsible for activation of NEK6 and NEK7 during mitosis. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 102-138 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |