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NUAK2, active, GST tagged human

SIGMA/SRP5054 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: DKFZP434J037; DKFZp686F01113; FLJ90349; SNARK

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-SRP5054-10UG 10 µg
 $575.00
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SDS-PAGE NUAK2, active, GST tagged, human: Cat. No. SRP5054: Purity was determined by densitometry. Molecular weight ~110 kDa. See CofA for lot-specific data.
Specific Activity NUAK2, active, GST tagged, human: Cat. No. SRP5054: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.

 

assay ≥70% (SDS-PAGE)
form buffered aqueous glycerol solution
mol wt ~110 kDa
NCBI accession no. NM_030952 
product line PRECISIO® Kinase
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
specific activity 68-92 nmol/min·mg
storage temp. −70°C
General description: NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2. NUAK2 is activated by muscle contraction and is a unique mediator of contraction-stimulated glucose transport in skeletal muscle. NUAK2 is involved in cellular stress responses linked to obesity and type 2 diabetes. NUAK2 shows kinase activity against a synthetic test peptide and the activity in keratinocytes is increased by AMP and 5-amino-4-imidazolecarboxamide riboside, implying that AMPK kinase-dependent pathway can activate NAUK2. Glucose deprivation also increases NAUK2 activity in baby hamster kidney fibroblasts.
Legal Information: PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form: Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note: after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 68-92 nmol/min·mg
Storage Temp. −70°C
UNSPSC 12352204

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