PDE6A (31-end), active, GST tagged human
SIGMA/SRP5058 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CGPR-A
Product Type: Chemical
SDS-PAGE PDE6A (31-end), active, GST tagged, human: Cat. No. SRP5058: Purity was determined by densitometry. Molecular weight ~120 kDa. See CofA for lot-specific data.
Specific Activity PDE6A (31-end), active, GST tagged, human: Cat. No. SRP5058: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~120 kDa |
| NCBI accession no. | NM_000440   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 32-44 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PDE6A encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A and is expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 32-44 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |