PKC ε , active, GST tagged human
SIGMA/SRP5067 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC125656; MGC125657; PRKCE; nPKC-ε
Product Type: Chemical
SDS-PAGE PKC epsilon , active, GST tagged, human: Cat. No. SRP5067: Purity was determined by densitometry. Molecular weight ~110 kDa. See CofA for lot-specific data.
Specific Activity PKC epsilon , active, GST tagged, human: Cat. No. SRP5067: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~110 kDa |
| NCBI accession no. | NM_005400   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 1033-1398 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PKCε is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can phosphorylate a wide variety of protein targets known to be involved in diverse cellular signaling pathways. PKCε is involved in many different cellular functions, such as neuron channel activation , cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may control anxiety-like behavior. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 1033-1398 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |