SIK (1-303), active, His tagged human
SIGMA/SRP5085 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MSK; SIK1; SNF1LK
Product Type: Chemical
SDS-PAGE SIK (1-303), active, His tagged, human: Cat. No. SRP5085: Purity was determined by densitometry. Molecular weight ~36 kDa. See CofA for lot-specific data.
Specific Activity SIK (1-303), active, His tagged, human: Cat. No. SRP5085: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~36 kDa |
| NCBI accession no. | NM_173354   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 296-401 nmol/min·mg |
| storage temp. | −70°C |
| General description: | SIK is a protein kinase that is involved in regulating AMPK-related kinases. SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane. This results in an increase in intracellular sodium in intact mammalian cells. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 296-401 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |