SLK, active, GST tagged human
SIGMA/SRP5086 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: KIAA0204; LOSK; MGC133067; STK2; bA16H23.1; se20-9
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~180 kDa |
NCBI accession no. | AB002804 |
product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 3.9-5.3 nmol/min·mg |
storage temp. | −70°C |
General description: | SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome. Inhibition of SLK activity by dominant-negative mutant or RNAi leads to unfocused microtubule arrangement indicating that SLK is needed for microtubule organization and for the proper localization of Golgi complex. Expression of v-Src can down-regulate SLK activity through the involvement of CK2 which can directly phosphorylate and inhibit SLK activity whereas inhibition of CK2 in v-Src-transformed cells results in normal SLK activity. CK2 and SLK can be co-localized in fibroblasts spreading on fibronectin-coated substrates. |
Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 3.9-5.3 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352202 |