TAK1-TAB1 (1-303), (437-end), active, GST tagged human
SIGMA/SRP5088 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP3K7; MGC57664; TAK1: MAP3K7
Product Type: Chemical
SDS-PAGE TAK1-TAB1 (1-303), (437-end), active, GST tagged, human: Cat. No. SRP5088: Purity was determined by densitometry. Molecular weight ~74 kDa. See CofA for lot-specific data.
Specific Activity TAK1-TAB1 (1-303), (437-end), active, GST tagged, human: Cat. No. SRP5088: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~74 kDa |
| NCBI accession no. | NM_003188   |
| NM_006116 |
|
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 8-12 nmol/min·mg |
| storage temp. | −70°C |
| General description: | TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (NfκB). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8. Thymocytes lacking TAK1 fail to activate NfκB and JNK and are prone to apoptosis upon stimulation. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 8-12 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |