TIE2 (771-end), active, GST tagged human
SIGMA/SRP5092 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CD202B; TEK; VMCM; VMCM1
Product Type: Chemical
SDS-PAGE TIE2 (771-end), active, GST tagged, human: Cat. No. SRP5092: Purity was determined by densitometry. Molecular weight ~61 kDa. See CofA for lot-specific data.
Specific Activity TIE2 (771-end), active, GST tagged, human: Cat. No. SRP5092: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~61 kDa |
| NCBI accession no. | NM_000459   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 10-14 nmol/min·mg |
| storage temp. | −70°C |
| General description: | TIE 2 or TEK is a receptor tyrosine kinase that is expressed principally on vascular endothelium. Disrupting TIE 2 function in mice results in embryonic lethality with defects in embryonic vasculature, suggesting a role in blood vessel maturation and maintenance. Angiopoietin-1 is a secreted growth factor that binds to and activates the TIE 2 receptor tyrosine kinase. SHP2 and GRB2 are recruited to the activated TIE 2 kinase domain and are part of the cellular responses that mediate TIE 2 function. TIE 2 expression is upregulated in the endothelium of vascular "hot spots" in human breast cancer specimens. However, TIE 2 is also overexpressed in areas of active angiogenesis in normal tissues. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 10-14 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |