ULK1 (1-649), active, GST tagged human
SIGMA/SRP5096 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: ATG1; FLJ38455; UNC51; Unc51.1
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~125 kDa |
NCBI accession no. | BC111603 |
product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 46-62 nmol/min·mg |
storage temp. | −70°C |
Application: | GST-ULK1 has been used in in vitro kinase assay. |
Biochem/physiol Actions: | Unc-51 like autophagy activating kinase 1 (ULK1) protein kinase plays a crucial role in the control of autophagy initiation. This protein is responsible for activating a cascade of signaling pathways which results in the formation of phagosomes. ULK1 is also required for the control of the expression of key interferon-stimulated genes (ISGs). |
General description: | Unc-51 like autophagy activating kinase 1 (ULK1) is a serine/threonine protein kinase which forms a component of the ULK1 protein kinase complex. This protein complex also contains the adaptor proteins ATG (autophagy-related) 13, RB1CC1/FIP200 (RB1-inducible coiled-coil 1), and ATG101. The gene encoding this protein is localized on human chromosome 12q24.33. |
Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 46-62 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |