ULK3, active, His tagged human
SIGMA/SRP5098 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: DKFZP434C131
Product Type: Chemical
SDS-PAGE ULK3, active, His tagged, human: Cat. No. SRP5098: Purity was determined by densitometry. Molecular weight ~51 kDa. See CofA for lot-specific data.
Specific Activity ULK3, active, His tagged, human: Cat. No. SRP5098: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~51 kDa |
| NCBI accession no. | BC157884   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 195-265 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins. ULK3 participates in cell cycle checkpoint and prevents the progression of cell division prior to complete chromosome separation. Overexpression of ULK3 stimulates autophagy through fibroblast activation. |
| General description: | The ULK3 (unc-51 like kinase 3) gene is mapped to human chromosome 15q24.1. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 195-265 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |