YES1, active, GST tagged human
SIGMA/SRP5100 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HsT441; P61-YES; Yes; c-yes
Product Type: Chemical
SDS-PAGE YES1, active, GST tagged, human: Cat. No. SRP5100: Purity was determined by densitometry. Molecular weight ~91 kDa. See CofA for lot-specific data.
Specific Activity YES1, active, GST tagged, human: Cat. No. SRP5100: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~91 kDa |
| NCBI accession no. | NM_005433   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 157-213 nmol/min·mg |
| storage temp. | −70°C |
| General description: | YES1 is the cellular homolog of the Yamaguchi sarcoma virus oncogene that has tyrosine kinase activity and belongs to the SRC family. YES1 lies in close proximity to thymidylate synthase gene on chromosome 18 and chromosome 22. The activation of YES1 may play a significant role in the malignant transformation of hepatocytes, and is important for maintaining embryonic stem cells in an undifferentiated state. YES1 is a useful marker to detect early-stage hepatocellular carcinoma, and it plays a key role in the tumorigenesis and metastasis of gastric cancer. YES1 induction results in increased cancer cell motility suggesting that YES1 may promote cancer spread and metastasis rather than tumor growth. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 157-213 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |