P300 (1283-1673), GST tagged human
SIGMA/SRP5110 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: EP300; KAT3B
CAS Number: 2226028-39-1
Product Type: Chemical
SDS-PAGE P300 (1283-1673), GST tagged, human: Cat. No. SRP5110: Purity was determined by densitometry. Molecular weight ~74 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~74 kDa |
| NCBI accession no. | NM_001429   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| General description: | P300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. P300 is related by sequence to CBP and like CBP can stimulate transcription through activation of CREB. The P300 activity is specifically inhibited by the adenovirus oncoprotein E1A. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. P300 is a components of DRAF1 (double-stranded RNA-activated factor-1), a positive regulator of interferon-stimulated gene transcription that functions as a direct response to viral infection. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |