RhoA, His tagged human
SIGMA/SRP5127 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: ARH12; ARHA; RHO12; RHOH12
Product Type: Chemical
SDS-PAGE RhoA, His tagged, human: Cat. No. SRP5127: Purity was determined by densitometry. Molecular weight ~23 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~23 kDa |
| NCBI accession no. | NM_001664   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | RhoA, His tagged human has been used for in vitro kinase assay. |
| Biochem/physiol Actions: | Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization. |
| General description: | Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31. |
| Physical form: | Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |