SMAD3, GST tagged human
SIGMA/SRP5132 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: DKFZP586N0721; DKFZp686J10186; HSPC193; HsT17436; JV15-2; MADH3; MGC60396
CAS Number: 184379-52-0
Product Type: Chemical
SDS-PAGE SMAD3, GST tagged, human: Cat. No. SRP5132: Purity was determined by densitometry. Molecular weight ~77 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~77 kDa |
| NCBI accession no. | NM_005902   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| General description: | SMAD3 is a direct mediator of transcriptional activation by the TGFβ receptor. The activity of SMAD3 is regulated by the TGFβ receptors, and SMAD3 is phosphorylated and associated with the ligand-bound receptor complex. TGFβ stimulation leads to phosphorylation and activation of SMAD3, which form a complex with SMAD4 that accumulate in the nucleus and regulate transcription of target genes such as CDK inhibitor. SMAD3 containing a C-terminal truncation acts as a dominant-negative inhibitor of the normal TGFβ response. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |