Tubulin, GST tagged human
SIGMA/SRP5148 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: M40; MGC117247; MGC16435; OK/SW-cl.56; TUBB; TUBB5
CAS Number: 1622402-26-9
Product Type: Chemical
SDS-PAGE Tubulin, GST tagged, human: Cat. No. SRP5148: Purity was determined by densitometry. Molecular weight ~76 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~76 kDa |
| NCBI accession no. | NM_178014   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | GST tagged tubulin has been used in Western blotting and in mass spec as a control. |
| Biochem/physiol Actions: | Tubulin-β 1 (TUBB1) interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. It has a role in the formation of the cytoskeleton. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance. |
| General description: | Tubulin-β 1 (TUBB1) protein is a major constituent of microtubules. Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both α and β tubulin monomers. It is found in hematopoietic cells and platelet marginal bands. The gene encoding TUBB1 is localized on human chromosome 6p21. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |