Vimentin, His tagged human
SIGMA/SRP5150 - recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: FLJ36605; VIM
CAS Number: 414913-88-5
Product Type: Chemical
SDS-PAGE Vimentin, His tagged, human: Cat. No. SRP5150: Purity was determined by densitometry. Molecular weight ~60 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥90% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~60 kDa |
| NCBI accession no. | NM_003380   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Vimentin, His tagged human has been used in the glass surface immobilization in anti-vimentin binding in force measurement by atomic force microscopy (AFM). |
| Biochem/physiol Actions: | Vimentin (VIM) stabilizes cytoskeletal interactions and is crucial for the placement of organelles in the cytosol. It modulates apoptosis in lymphocytes and is involved in the pathogenesis of inflammatory diseases VIM may serve as a potential marker for sepsis diagnosis. It is also essential for lens integrity maintenance. |
| Biochem/physiol Actions: | Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells. Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions |
| General description: | Vimentin belongs to the intermediate filament family of proteins. It is a key structural component of eukaryotic cells and is associated with lymphocytes and neutrophils. Vimentin exists as a tripartite domain structure. It is expressed in mesenchymal cells. The vimentin gene is mapped to human chromosome 10p13. |
| Physical form: | Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥90% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |