4EBP1, His tagged human
SIGMA/SRP5162 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: BP-1; EIF4EBP1; MGC4316; PHAS-I
CAS Number: 185703-54-2
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | human |
form | buffered aqueous glycerol solution |
mol wt | ~18 kDa |
NCBI accession no. | NM_004095 |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
storage temp. | −70°C |
Biochem/physiol Actions: | Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is involved in protein synthesis, cell growth and survival. In the unphosphorylated form, it suppresses the translation initiation of capped mRNAs, whereas in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eukaryotic translation initiation factor 4E (eIF4E) and this results in cap-dependent translation. The protein is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, E1F4EBP1 interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21. |
General description: | Eukaryotic translation initiation factor 4E binding protein 1 (E1F4EBP1) is part of the translation repressor protein family. The gene encoding it is localized on human chromosome 8p11-p12. |
Physical form: | Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H360D |
Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Purity | ≥70% (SDS-PAGE) |
Storage Temp. | −70°C |
UNSPSC | 12352202 |