Catenin β, GST tagged human
SIGMA/SRP5172 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CTNNB; FLJ25606
CAS Number: 847964-78-7
Product Type: Chemical
SDS-PAGE Catenin, GST tagged, human: Cat. No. SRP5172: Purity was determined by densitometry. Molecular weight ~115 kDa. See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~115 kDa |
| NCBI accession no. | NM_001904   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| storage temp. | −70°C |
| Application: | Catenin β, GST tagged human has been used: • as a component of protein kinase buffer for in vitro kinase assay • in in vitro citrullination assay to characterize β-catenin citrullination • to perform differentiation factor screening |
| Biochem/physiol Actions: | β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome. APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas. The protein has a role in homeostasis and development. |
| General description: | β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins associate with E-cadherin at cellular junctions. CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats and a 100 amino acid C-terminal domain. The gene encoding β-catenin is localized on human chromosome 3p22.1. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |