LC20, GST tagged from mouse
SIGMA/SRP5203 - recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC3505; MLC2; MRLC1; MYL9; MYRL2
CAS Number: 562031-72-5
Product Type: Chemical
SDS-PAGE LC20, GST tagged, mouse: Cat. No. SRP5203: Purity was determined by densitometry. Molecular weight ~45 kDa. See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| biological source | mouse |
| form | buffered aqueous glycerol solution |
| mol wt | ~45 kDa |
| NCBI accession no. | BC055439   |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| storage temp. | −70°C |
| General description: | LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin. |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |