EIF2AK4 (192-1024), active, GST tagged human
SIGMA/SRP5218 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: GCN2; KIAA1338
Product Type: Chemical
SDS-PAGE EIF2AK4 (192-1024), active, GST tagged, human: Cat. No. SRP5218: Purity was determined by densitometry. Molecular weight ~132 kDa. See CofA for lot-specific data.
Specific Activity EIF2AK4 (192-1024), active, GST tagged, human: Cat. No. SRP5218: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~132 kDa |
| NCBI accession no. | NM_001013703   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 11.9-16.1 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Eukaryotic translation initiation factor 2-α kinase 4 (EIF2AK4) is responsible for phosphorylation of the a subunit of the eukaryotic initiation factor 2 (eIF2α). The EIF2AK4 eIF2-α kinase regulates fatty-acid homeostasis in the liver during deprivation of an essential amino acid. The protein p58IPK inhibits EIF2AK4 activity. EIF2AK4 is linked to pulmonary veno-occlusive disease (PVOD) development and its pathway is associated with diseases such as cancer and Alzheimer′s disease. The protein affects many aspects of cellular physiology in response to a number of stresses such as starvation. Mutation in EIF2AK4 may cause autosomal-recessive pulmonary capillary hemangiomatosis (PCH). EIF2AK4 causes tumor growth and angiogenesis by expression of vascular endothelial growth factor (VEGF). |
| General description: | Eukaryotic translation initiation factor 2-α kinase 4 (EIF2AK4), also known as GCN2 is an enzyme encoded by EIF2AK4 gene in humans. In mouse, EIF2AK4 is widely expressed with prominent expression in liver and brain. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 11.9-16.1 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |