MLK2 (1-446), active, GST tagged human
SIGMA/SRP5235 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP3K10; MST
Product Type: Chemical
SDS-PAGE MLK2 (1-446), active, GST tagged, human: Cat. No. SRP5235: Purity was determined by densitometry. Molecular weight ~76 kDa. See CofA for lot-specific data.
Specific Activity MLK2 (1-446), active, GST tagged, human: Cat. No. SRP5235: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~76 kDa |
| NCBI accession no. | NM_002446   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 41-55 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8 JNK and MKK4/SEK1. MLK2 itself can be phoshorylated, and activated by JNK kinases. MLK2 is also known as mitogen-activated protein kinase kinase kinase 10 (MAP3K10). MLK2 functions preferentially on the JNK signaling pathway which is involved in nerve growth factor (NGF) induced neuronal apoptosis. MLK2 was isolated as a cDNA fragment from MKN28 gastric cancer cell library using degenerate PCR. MLK2 is highly expressed in brain, skeletal muscle, and testis. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 41-55 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |