NUAK1, active, GST tagged human
SIGMA/SRP5237 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: ARK5; KIAA0537
CAS Number: 2158240-34-5
Product Type: Chemical
SDS-PAGE NUAK1, active, GST tagged, human: Cat. No. SRP5237: Purity was determined by densitometry. Molecular weight ~122 kDa. See CofA for lot-specific data.
Specific Activity NUAK1, active, GST tagged, human: Cat. No. SRP5237: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~122 kDa |
| NCBI accession no. | NM_014840   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 42-58 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) facilitates cancer cell migration activity. It plays a major role in tumorigenesis, cancer invasion, and metastasis. This tumor cell survival factor is activated by AKT. It serves as an ATM kinase under conditions of nutrient starvation. Overexpression of ARK5 is observed in human ovarian cancer. It is also overexpressed in breast cancer, colorectal carcinoma, and hepatocellular carcinoma (HCC). ARK5 knockdown prevents the aggressive features of SKOV3 cells. NUAK1 is involved in tolerance to glucose starvation and suppresses Fas-induced apoptosis by phosphorylation of caspase 6 (CASP6), thus suppressing the activation of the caspase and the subsequent cleavage of CFLAR. |
| General description: | NUAK family kinase 1(NUAK1)/AMPK-related protein kinase-5 (ARK5) belongs to the human AMP-activated protein kinases (AMPKs) family. It is highly expressed in the heart and brain, and at lower levels in skeletal muscle, kidney, ovary, placenta, lung, and liver. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 42-58 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |