CK1 γ 3, active, GST tagged human
SIGMA/SRP5241 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: CSNK1G3
Product Type: Chemical
assay | ≥70% (SDS-PAGE) |
form | buffered aqueous glycerol solution |
mol wt | ~73 kDa |
NCBI accession no. | NM_001031812 |
product line | PRECISIO® Kinase |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 17-23 nmol/min·mg |
storage temp. | −70°C |
General description: | CK1gamma3 is a member of the CK1 family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CK1gamma3 is a ubiquitously expressed protein kinase found in the nuclei, cytoplasm and membrane fractions of eukaryotic cells. CK1gamma3 preferentially phosphorylates acidic substrates using ATP as a phosphate donor. The kinase domain of CKI isoforms have been shown to associate with protein kinase C-potentiated inhibitor protein of 17kDa, called CPI-17. CPI-17 specifically inhibits myosin light chain phosphatase and this effect is potentiated when it is phosphorylated on Thr-38 by protein kinases such as the CK1 isoforms. |
Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 17-23 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |