MLK1 (1-433), active, GST tagged human
SIGMA/SRP5244 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP3K9; MEKK9; PRKE1
Product Type: Chemical
SDS-PAGE MLK1 (1-433), active, GST tagged, human: Cat. No. SRP5244: Purity was determined by densitometry. Molecular weight ~77 kDa. See CofA for lot-specific data.
Specific Activity MLK1 (1-433), active, GST tagged, human: Cat. No. SRP5244: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~77 kDa |
| NCBI accession no. | NM_033141   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 99-133 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MLK1 or Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway. The catalytic domain of MLK1 has amino acid sequence similarity to both the tyr-specific and the ser/thr-specific kinase classes. In addition, MLK1 contain 2 leu/ile-zipper motifs and a basic sequence near its C-termini. MLK1 is threonine (and possibly serine) phosphorylated at multiple sites in the activation loop, with phosphorylation of Thr312 required for full activation. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 99-133 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |