MLK3 (1-488), active, GST tagged human
SIGMA/SRP5245 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP3K11; MGC17114; MLK-3; MLK3; PTK1; SPRK
CAS Number: 153190-46-6
Product Type: Chemical
SDS-PAGE MLK3 (1-488), active, GST tagged, human: Cat. No. SRP5245: Purity was determined by densitometry. Molecular weight ~83 kDa. See CofA for lot-specific data.
Specific Activity MLK3 (1-488), active, GST tagged, human: Cat. No. SRP5245: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~83 kDa |
| NCBI accession no. | NM_002419   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 8-11 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MLK3 or Mixed-Lineage Kinase 3 is a mitogen-activated protein kinase kinase kinase capable of preferentially activating MAPK8/JNK kinase and functions as a positive regulator of JNK signaling pathway. MLK3 can directly phosphorylate and activate IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. MLK3 is a signal-integrating kinase with conventional MAP3K catalytic activity and additional noncatalytic functions that contribute to RAF/ERK signalling. MLK3 is a component of the BRAF/RAF1 complex and is required for integrity of the complex and for activation of ERK by the complex. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 8-11 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |