PAK1, active, GST tagged human
SIGMA/SRP5246 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC130000; MGC130001; PAKα
CAS Number: 177893-51-5
Product Type: Chemical
SDS-PAGE PAK1, active, GST tagged, human: Cat. No. SRP5246: Purity was determined by densitometry. Molecular weight ~95 kDa. See CofA for lot-specific data.
Specific Activity PAK1, active, GST tagged, human: Cat. No. SRP5246: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~95 kDa |
| NCBI accession no. | NM_002576   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 63-85 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | p21-activated kinases (PAKs) are serine/threonine kinases that are activated by and are effectors of small GTPases, cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate (RAC). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix. The protein has a role in numerous cellular processes and has been shown to be expressed in squamous non-small cell lung carcinomas. |
| General description: | p21 (RAC1) activated kinase 1 (PAK1) is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. The gene encoding this protein is localized on human chromosome 11q13. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 63-85 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |