PDHK2, active, GST tagged human
SIGMA/SRP5248 - PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: PDK2
Product Type: Chemical
SDS-PAGE PDHK2, active, GST tagged, human: Cat. No. SRP5248: Purity was determined by densitometry. Molecular weight ~70 kDa. See CofA for lot-specific data.
Specific Activity PDHK2, active, GST tagged, human: Cat. No. SRP5248: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot-specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~70 kDa |
| NCBI accession no. | NM_002611   |
| product line | PRECISIO® Kinase |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 6.4-8.6 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | PDHK2 or Pyruvate Dehydrogenase Kinase 2 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDHK2 is a primary PPARbeta/delta target gene in humans which underlines the importance of PPAR in the control of metabolism. There are two candidate PPAR response elements in the PDHK2 gene. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol. |
| Preparation Note: | after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 6.4-8.6 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |