PKC-α, active, GST tagged from Xanopus> sp.
SIGMA/SRP5251 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC82897; PRKCA
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | Xenopus sp. |
form | buffered aqueous glycerol solution |
mol wt | ~103 kDa |
NCBI accession no. | BC078065 ![]() ![]() |
product line | PRECISIO® |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 186-290 nmol/min·mg |
storage temp. | −70°C |
Biochem/physiol Actions: | Protein kinase C-α (PKC-α) has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint and cell volume control. It has been identified as a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. |
General description: | Protein kinase C-α (PKC-α) is a member of the PKC family of serine- and threonine-specific protein kinases that can be activated by calcium and the secondary messenger diacylglycerol. The protein is expressed in the dorsal ectoderm of Xenopus. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 186-290 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |