PKC-α, active, GST tagged from Xanopus sp.

SIGMA/SRP5251 - PRECISIO^®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: MGC82897; PRKCA

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity

45-SRP5251-10UG 10 µg
$370.00
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SDS-PAGE PKC-α, active, GST tagged, xenopus: Cat. No. SRP5251: Purity was determined by densitometry. Molecular weight ~103 kDa. See CofA for lot specific data.

Specific Activity PKC-α, active, GST tagged, xenopus: Cat. No. SRP5251: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.

Properties

Descriptions

Safety Info.

Basic Data

application(s) cell analysis

assay ≥70% (SDS-PAGE)

biological source Xenopus sp.

form buffered aqueous glycerol solution

mol wt ~103 kDa

NCBI accession no. BC078065

product line PRECISIO^®

recombinant expressed in baculovirus infected Sf9 cells

shipped in dry ice

specific activity 186-290 nmol/min·mg

storage temp. −70°C

Biochem/physiol Actions: Protein kinase C-α (PKC-α) has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint and cell volume control. It has been identified as a fundamental regulator of cardiac contractility and Ca²⁺ handling in myocytes.

General description: Protein kinase C-α (PKC-α) is a member of the PKC family of serine- and threonine-specific protein kinases that can be activated by calcium and the secondary messenger diacylglycerol. The protein is expressed in the dorsal ectoderm of Xenopus.

Legal Information: PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Physical form: Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Preparation Note: After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

RIDADR NONH for all modes of transport

WGK Germany WGK 1

Flash Point(F) Not applicable

Flash Point(C) Not applicable

Purity ≥70% (SDS-PAGE)

activity specific activity: 186-290 nmol/min·mg

Storage Temp. −70°C

UNSPSC 12352200

application(s)	cell analysis
assay	≥70% (SDS-PAGE)
biological source	Xenopus sp.
form	buffered aqueous glycerol solution
mol wt	~103 kDa
NCBI accession no.	BC078065
product line	PRECISIO^®
recombinant	expressed in baculovirus infected Sf9 cells
shipped in	dry ice
specific activity	186-290 nmol/min·mg
storage temp.	−70°C

Biochem/physiol Actions:	Protein kinase C-α (PKC-α) has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint and cell volume control. It has been identified as a fundamental regulator of cardiac contractility and Ca²⁺ handling in myocytes.
General description:	Protein kinase C-α (PKC-α) is a member of the PKC family of serine- and threonine-specific protein kinases that can be activated by calcium and the secondary messenger diacylglycerol. The protein is expressed in the dorsal ectoderm of Xenopus.
Legal Information:	PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC
Physical form:	Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note:	After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥70% (SDS-PAGE)
activity	specific activity: 186-290 nmol/min·mg
Storage Temp.	−70°C
UNSPSC	12352200

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