SIRT1, active, His tagged human
SIGMA/SRP5270 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: SIR2L1
CAS Number: 1391438-73-5
Product Type: Chemical
SDS-PAGE SIRT1, active, His tagged, human: Cat. No. SRP5270: Purity was determined by densitometry. Molecular weight ~84 kDa. See CofA for lot specific data.
Specific Activity SIRT1, active, His tagged, human: Cat. No. SRP5270: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | genomic analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~84 kDa |
| NCBI accession no. | NM_012238   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 1573-2415 RLU/min·ng |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Sirtuin 1 (SIRT1) plays an important role in neuronal proliferation and vascular cells. It also plays an athero-protective role. Mutations in the SIRT1 gene affects the metabolism, cholesterol and fat deposition. SIRT1 has the ability to intermediate the insulin resistance through an inflammatory process, reactive oxygen species, gluconeogenesis and adiponectin level. It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation. |
| General description: | Sirtuin 1 (SIRT1) is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. SIRT1 is also called as NAD-dependent deacetylase sirtuin-1. This gene is located on human chromosome 10q21.3. |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 1573-2415 RLU/min·ng |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |