SIRT7, active, His tagged human
SIGMA/SRP5274 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC126840; MGC126842; SIR2L7
CAS Number: 1391453-59-0
Product Type: Chemical
SDS-PAGE SIRT7, active, His tagged, human: Cat. No. SRP5274: Purity was determined by densitometry. Molecular weight ~46 kDa. See CofA for lot specific data.
Specific Activity SIRT7, active, His tagged, human: Cat. No. SRP5274: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | genomic analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~46 kDa |
| NCBI accession no. | NM_016538   |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 112-139 RLU/min·ng |
| storage temp. | −70°C |
| General description: | SIRT7 is a member of the class IV of sirtuin family of proteins which are homologs to the yeast Sir2 protein and play a role in cell differentiation, proliferation, apoptosis, metabolism, and senescence. SIRT7 associates with active rRNA genes and histones. Overexpression of SIRT7 increases pol I-mediated transcription whereas knockdown of SIRT7 or inhibition of its catalytic activity results in decreased association of pol I with rDNA and reduced pol I transcription. Depletion of SIRT7 stops cell proliferation and triggers apoptosis. SIRT7 deacetylates p53 and increases cellular resistance to cytotoxic and oxidative stress. |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 112-139 RLU/min·ng |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |