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SIRT3, active, GST tagged human

SIGMA/SRP5275 - recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: SIR2L3

CAS Number: 1391449-26-5
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP5275-10UG 10 µg
$616.00
1/EA
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SDS-PAGE SIRT3, active, GST tagged, human: Cat. No. SRP5275: Purity was determined by densitometry. Molecular weight ~66 kDa. See CofA for lot specific data.
Specific Activity SIRT3, active, GST tagged, human: Cat. No. SRP5275: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.

 

application(s) cell analysis
assay ≥70% (SDS-PAGE)
biological source human
form buffered aqueous glycerol solution
mol wt ~66 kDa
NCBI accession no. NM_012239 
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
specific activity 123-196 RLU/min·ng
storage temp. −70°C
General description: SIRT3 is a member of the class I of the Sirtuin family that is characterized by a Sirtuin core domain. Sirtuin are grouped into four classes and are homologs to the Sir2 protein. SIRT3 is known to regulate the epigenetic gene silencing and suppresses recombination of rDNA. SIRT3 may function as an intracellular regulatory protein with mono-ADP-ribosyltransferase activity that is essential for its silencing function. SIRT3 regulates and maintains basal ATP levels in the cell. SIRT3 also modulates mitochondrial intermediary metabolism and fatty acid use during fasting (3).
Physical form: Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note: After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 123-196 RLU/min·ng
Storage Temp. −70°C
UNSPSC 12352202

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