PI3K (p110-α/p85-α), active, His tagged from mouse and human
SIGMA/SRP5280 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: p110-alpha; p85-alpha
CAS Number: 115926-52-8
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | human |
mouse | |
form | buffered aqueous glycerol solution |
mol wt | ~111 kDa (p110α) |
~86 kDa (p85α) | |
NCBI accession no. | NM_008839 |
product line | PRECISIO® |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 64-97 nmol/min·mg |
storage temp. | −70°C |
General description: | The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling. |
Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
Symbol | GHS07,GHS08 |
Signal word | Danger |
Hazard statements | H315 - H319 - H360D |
Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
Hazard Codes | T |
Risk Statements | 61 |
Safety Statements | 53-45 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 64-97 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |