PI3K (p110-α/p85-α), active, His tagged from mouse and human
SIGMA/SRP5280 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: p110-alpha; p85-alpha
CAS Number: 115926-52-8
Product Type: Chemical
SDS-PAGE PI3K (p110-α/p85-α), active, His tagged, mouse and human: Cat. No. SRP5280: Purity was determined by densitometry. Molecular weight of p110β is ~111 kDa, and p85α is ~86 kDa. See CofA for lot specific data.
Specific Activity PI3K (p110-α/p85-α), active, His tagged, mouse and human: Cat. No. SRP5280: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| mouse | |
| form | buffered aqueous glycerol solution |
| mol wt | ~111 kDa (p110α) |
| ~86 kDa (p85α) | |
| NCBI accession no. | NM_008839   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 64-97 nmol/min·mg |
| storage temp. | −70°C |
| General description: | The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 64-97 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |