ERK1, active, GST tagged human
SIGMA/SRP5282 - PRECISIO®, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HS44KDAP; HUMKER1A; MAPK3; MGC20180; P44ERK1; P44MAPK; PRKM3
CAS Number: 137632-07-6
Product Type: Chemical
SDS-PAGE ERK1, active, GST tagged, human: Cat. No. SRP5282: Purity was determined by densitometry. Molecular weight ~72 kDa. See CofA for lot specific data.
Specific Activity ERK1, active, GST tagged, human: Cat. No. SRP5282: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~72 kDa |
| NCBI accession no. | NM_002746   |
| product line | PRECISIO® |
| recombinant | expressed in E. coli |
| shipped in | dry ice |
| specific activity | 672-1008 nmol/min·mg |
| storage temp. | −70°C |
| General description: | ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), also known as mitogen-activated protein kinase 3 (MAPK3) which are activated in response to numerous growth factors and cytokines. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta). ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. ERK activation affects the axonal growth by phosphorylation of microtubule-associated proteins and neurofilaments. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 672-1008 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |