HPK1, active, GST tagged human
SIGMA/SRP5284 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MAP4K1
CAS Number: 182762-12-5
Product Type: Chemical
SDS-PAGE HPK1, active, GST tagged, human: Cat. No. SRP5284: Purity was determined by densitometry. Molecular weight ~65 kDa. See CofA for lot specific data.
Specific Activity HPK1, active, GST tagged, human: Cat. No. SRP5284: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~65 kDa |
| NCBI accession no. | NM_007181   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 106-166 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Hematopoietic progenitor kinase 1 (HPK1) acts as a tissue-specific upstream activator for the stress-activated protein kinases (SAPK)/Jun amino-terminal kinases (JNK) signaling pathway. By phosphorylating SH2 domain-containing leukocyte protein of 76 kDa (SLP-76), HPK1 effectively blocks the T cell receptor (TCR) signaling. HPK1 is implicated in the inflammatory response and in the pathogenesis of systemic lupus erythematosus (SLE). It mediates the degradation of IκB and nuclear factor-κB (NF-κB) activation by activating inhibitor of nuclear factor-κB (IκB) kinase (IKK) complex. |
| General description: | Hematopoietic progenitor kinase 1 (HPK1) belongs to the Ste20 serine/threonine kinase superfamily. It comprises a C-terminal citron homology (CNH) domain and a total of 13 tyrosines as potential phosphorylation residues. The HPK1 gene is mapped to human chromosome 19q13. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 106-166 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |