NLK, active, GST tagged human
SIGMA/SRP5285 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: DKFZp761G1211; FLJ21033
Product Type: Chemical
SDS-PAGE NLK, active, GST tagged, human: Cat. No. SRP5285: Purity was determined by densitometry. Molecular weight ~84 kDa. See CofA for lot specific data.
Specific Activity NLK, active, GST tagged, human: Cat. No. SRP5285: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~84 kDa |
| NCBI accession no. | NM_016231   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 1.9-2.9 nmol/min·mg |
| storage temp. | −70°C |
| General description: | NLK (also known as nemo-like kinase) is a member of the serine/threonine protein kinase that is encoded by the NLK gene. NLK plays a role in cell fate determination and is required for the differentiation of bone marrow stromal cells. NLK also suppresses a wide range of transcription factors such as CREB by phosphorylation of the co-activator CREBBP. NLK expression increases the number of apoptotic cells but does not induce obvious cell cycle arrest. NLK suppresses Notch signalling by interfering with formation of the Notch active transcriptional complex. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 1.9-2.9 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352202 |