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PIP5K1A, active, GST tagged human

SIGMA/SRP5292 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym: RP11-68I18.9

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SRP5292-10UG 10 µg
$520.00
1/EA
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SDS-PAGE PIP5K1A, active, GST tagged, human: Cat. No. SRP5292: Purity was determined by densitometry. Molecular weight ~86 kDa. See CofA for lot specific data.
Specific Activity PIP5K1A, active, GST tagged, human: Cat. No. SRP5292: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.

 

application(s) cell analysis
assay ≥70% (SDS-PAGE)
biological source human
form buffered aqueous glycerol solution
mol wt ~86 kDa
NCBI accession no. BC007833 
product line PRECISIO®
recombinant expressed in baculovirus infected Sf9 cells
shipped in dry ice
specific activity 947-1281 nmol/min·mg
storage temp. −70°C
General description: PIP5K1A or phosphatidylinositol-4-phosphate 5-kinase 1 alpha phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1 is required for Wnt3a-induced LRP6 phosphorylation at ser1490 in mammalian cells. Wnt3a stimulates the formation of phosphatidylinositol 4,5-bisphosphates through ′frizzled′ and ′dishevelled′, the latter of which directly interacts with and activated PIP5KI. PIP5K1A-mediated PIP production is crucial for HIV-1 entry and the early steps of infection in permissive lymphocytes. Overexpression of wild-type PI4P5K1A increases HIV-1 Env-mediated PIP production and enhances viral replication in primary lymphocytes.
Legal Information: PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form: Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note: After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
RIDADR NONH for all modes of transport
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥70% (SDS-PAGE)
activity specific activity: 947-1281 nmol/min·mg
Storage Temp. −70°C
UNSPSC 12352200

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