PI3K (p110-β/p85-β), active, His tagged human
SIGMA/SRP5295 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: p110-beta; p85-beta
CAS Number: 115926-52-8
Product Type: Chemical
SDS-PAGE PI3K (p110-β/p85-β), active, His tagged, human: Cat. No. SRP5295: Purity was determined by densitometry. Molecular weight of p110β is ~111 kDa, and p85β is ~88 kDa. See CofA for lot specific data.
Specific Activity PI3K (p110-β/p85-β), active, His tagged, human: Cat. No. SRP5295: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~111 kDa (p110β) |
| ~88 kDa (p85β) | |
| NCBI accession no. | NM_006219   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 22.4-33.6 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PI3K phosphorylate the 3-prime OH position of the inositol ring of inositol lipids which functions in signaling pathways that regulate cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kDa catalytic subunit and a 85-kDa adaptor subunit. PI3K plays a critical role in regulating the formation and stability of alpha-2B-beta-3 integrin adhesion bonds, which are necessary for shear force-induced platelet activation. PI3K sustains alpha-2B-beta-3 integrin activation and stabilized platelet aggregation by regulating both integrin-dependent calcium flux and Gi activation of RAP1B. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 22.4-33.6 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |