MET (M1250T), active, GST tagged human
SIGMA/SRP5302 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HGFR; RCCP2
Product Type: Chemical
SDS-PAGE MET (M1250T), active, GST tagged, human: Cat. No. SRP5302: Purity was determined by densitometry. Molecular weight ~81 kDa. See CofA for lot specific data.
Specific Activity MET (M1250T), active, GST tagged, human: Cat. No. SRP5302: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~81 kDa |
| NCBI accession no. | NM_000245   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 148-222 nmol/min·mg |
| storage temp. | −70°C |
| General description: | MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kDa (alpha) and 145 kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue. Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion. MET(M1250T) is one of the mutant forms of MET. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 148-222 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |