PIK3C3 (Vps34), active, GST tagged human
SIGMA/SRP5306 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: MGC61518; VPS34; hVps34
Product Type: Chemical
SDS-PAGE PIK3C3 (Vps34), active, GST tagged, human: Cat. No. SRP5306: Purity was determined by densitometry. Molecular weight ~128 kDa. See CofA for lot specific data.
Specific Activity PIK3C3 (Vps34), active, GST tagged, human: Cat. No. SRP5306: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~128 kDa |
| NCBI accession no. | NM_002647   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 78-216 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PIK3C3 or phosphoinositide-3-kinase is a member of the PI 3-kinase family that mediates the active diversion of proteins from the secretory pathway to vacuoles. PIK3C3 are involved in both receptor-mediated signal transduction and intracellular trafficking. In yeast, the vps34 gene product is a PI 3-kinase. PIK3C3 has a substrate specificity and ion requirements that are distinct from the other known mammalian PI 3-kinases. PIK3C3 mediate catalysis on membranes and suppress futile adenosine triphosphatase cycles. PIK3C3 appears to alternate between a closed cytosolic form and an open form on the membrane. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 78-216 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |