HIPK2, active, GST tagged from mouse
SIGMA/SRP5313 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: 1110014O20Rik; B230339E18Rik; Stank
Product Type: Chemical
application(s) | cell analysis |
assay | ≥70% (SDS-PAGE) |
biological source | mouse |
form | buffered aqueous glycerol solution |
mol wt | ~72 kDa |
NCBI accession no. | BC031904 ![]() ![]() |
product line | PRECISIO® |
recombinant | expressed in baculovirus infected Sf9 cells |
shipped in | dry ice |
specific activity | 88-132 nmol/min·mg |
storage temp. | −70°C |
General description: | HIPK2 is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors. HIPK2 enhances expression of p53 target genes that resulting in growth arrest and the enhancement of UV-induced apoptosis that could be inhibited by antisense to HIPK2. HIPK2 as a target for MDM2 mediated ubiquitin-dependent degradation that only occurred in growth-arresting conditions when MDM2 was efficiently induced by p53. The knockdown of HIPK2 using small interfering RNA reduced p53 binding and activation of p53R2 that resulting in impaired UV-induced DNA repair. |
Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥70% (SDS-PAGE) |
activity | specific activity: 88-132 nmol/min·mg |
Storage Temp. | −70°C |
UNSPSC | 12352200 |