PLK4, active, GST tagged human
SIGMA/SRP5315 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: SAK; STK18
CAS Number: 157482-37-6
Product Type: Chemical
SDS-PAGE PLK4, active, GST tagged, human: Cat. No. SRP5315: Purity was determined by densitometry. Molecular weight ~140 kDa. See CofA for lot specific data.
Specific Activity PLK4, active, GST tagged, human: Cat. No. SRP5315: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~140 kDa |
| NCBI accession no. | NM_014264   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 4.5-6.7 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells.The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 4.5-6.7 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |