RIPK3, active, GST tagged human
SIGMA/SRP5316 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: RIP3
CAS Number: 236750-39-3
Product Type: Chemical
SDS-PAGE RIPK3, active, GST tagged, human: Cat. No. SRP5316: Purity was determined by densitometry. Molecular weight ~96 kDa. See CofA for lot specific data.
Specific Activity RIPK3, active, GST tagged, human: Cat. No. SRP5316: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~96 kDa |
| NCBI accession no. | NM_006871   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 30-51 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Receptor interacting serine/threonine kinase 3 (RIPK3) activates necroptotic cell death and has a role in inflammatory signaling. The protein also functions in controlling viral infections, platelet functions, hemostasis and thrombosis. RIPK3 is highly expressed during cutaneous wound repair and also stimulates interleukin-1 β production. |
| General description: | Receptor interacting serine/threonine kinase 3 (RIPK3)/RIP3 is a serine/threonine kinase. It belongs to the receptor-interacting protein family. RIPK3 is characterized with a conserved amino-terminal kinase domain, which is necessary for necrosis. The gene RIPK3 is mapped to human chromosome 14. The encoded protein is composed of 518 amino acids. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 30-51 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |