PI3K (p110-α/p55-γ), active, His tagged human
SIGMA/SRP5319 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: p110-alpha; p55-gamma
Product Type: Chemical
SDS-PAGE PI3K (p110-α/p55-γ), active, His tagged, human: Cat. No. SRP5319: Purity was determined by densitometry. Molecular weight of p110α is ~111 kDa, and p55γ is ~61 kDa. See CofA for lot specific data.
Specific Activity PI3K (p110-α/p55-γ), active, His tagged, human: Cat. No. SRP5319: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| assay | ≥70% (SDS-PAGE) |
| form | buffered aqueous glycerol solution |
| mol wt | ~111 kDa (p110α) |
| ~61 kDa (p85γ) | |
| NCBI accession no. | NM_006218   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 11.2-16.8 nmol/min·mg |
| storage temp. | −70°C |
| General description: | PI3K (p110alpha/p55gamma) or Phosphatidylinositol 3-kinase is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position which serve as second messengers in growth signaling pathways. PI3K comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in the PI3K has been associated with insulin resistance and plays an important role in glucose homeostasis in vivo. PI3K also plays an essential role in the development and induction of mast cells in normal and pathogenic immune responses. |
| Legal Information: | PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 11.2-16.8 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |