EIF2AK1 (HRI), active, GST tagged human
SIGMA/SRP5321 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: HCR; HRI; KIAA1369
CAS Number: 1418571-40-0
Product Type: Chemical
SDS-PAGE EIF2AK1 (HRI), active, GST tagged, human: Cat. No. SRP5321: Purity was determined by densitometry. Molecular weight ~120 kDa. See CofA for lot specific data.
Specific Activity EIF2AK1 (HRI), active, GST tagged, human: Cat. No. SRP5321: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~120 kDa |
| NCBI accession no. | NM_014413   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 9.6-14.4 nmol/min·mg |
| storage temp. | −70°C |
| Biochem/physiol Actions: | Eukaryotic translation initiation factor 2-α kinase 1 (EIF2AK1) modulates protein synthesis. It acts at the level of translation initiation to downregulate protein synthesis in response to stress. The protein is activated by heme deficiency and other stimuli and is a major protein kinase that phosphorylates EIF2-α. EIF2AK1 is downregulated in the majority of ovarian cancers compared with normal ovarian tissues. It functions in iron homeostasis and may play a role in hemolytic and inflammatory anemia. |
| General description: | Eukaryotic translation initiation factor 2-α kinase 1 (EIF2AK1) or HRI is a kinase that can be inactivated by hemin. The gene encoding this protein is localized on human chromosome 7p22.1. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 9.6-14.4 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |