GRK3, active, GST tagged human
SIGMA/SRP5322 - PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym: ADRBK2; BARK2
CAS Number: 147171-37-7
Product Type: Chemical
SDS-PAGE GRK3, active, GST tagged, human: Cat. No. SRP5322: Purity was determined by densitometry. Molecular weight ~84 kDa. See CofA for lot specific data.
Specific Activity GRK3, active, GST tagged, human: Cat. No. SRP5322: Specific Activity was determined by activity assay (see datasheet for protocol). See CofA for lot specific data.
| application(s) | cell analysis |
| assay | ≥70% (SDS-PAGE) |
| biological source | human |
| form | buffered aqueous glycerol solution |
| mol wt | ~84 kDa |
| NCBI accession no. | NM_005160   |
| product line | PRECISIO® |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | dry ice |
| specific activity | 4.8-7.2 nmol/min·mg |
| storage temp. | −70°C |
| General description: | GRK3 or beta-adrenergic receptor kinase 2 specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. GRK3 is a member of the receptor kinase family that broadly serves to regulate receptor function. GRK3 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. GRK3 is highly expressed in lung, heart, and adipose tissue. Single nucleotide polymorphisms (SNPs) in the promoter region of GRK3 have been associated with bipolar disorder. |
| Legal Information: | PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol. |
| Preparation Note: | After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥70% (SDS-PAGE) |
| activity | specific activity: 4.8-7.2 nmol/min·mg |
| Storage Temp. | −70°C |
| UNSPSC | 12352200 |